Novel Methods Using an Arthrobacter sp. to Create Anaerobic Conditions for Biobutanol Production from Sweet Sorghum Juice by Clostridium beijerinckii

Biobutanol can be produced by Clostridia via an acetone–butanol–ethanol (ABE) fermentation under strictly anaerobic conditions. Oxygen-free nitrogen (OFN) gas is typically used to create conditions for ABE fermentations. However, this method not appropriate large-scale fermentations as it quite costly. The aim of work was study the feasibility butanol production from sweet sorghum juice (SSJ) Clostridium beijerinckii TISTR 1461 using various methods conditions, i.e., growth a aerobic bacterium, Arthrobacter sp., different and chemical sodium dithionite (SDTN) consume residual oxygen. SSJ containing 60 g/L total sugar supplemented with 1.27 (NH4)2SO4 substrate production. results showed that 0.25 mM SDTN could but in case, C.beijerinckii produce at concentration (PB) only 8.51 productivity (QB) 0.10 g/L·h. sp. BCC 72131 also Mixed cultures created inoculating 4 h after Arthrobacter. This gave PB 10.39 QB 0.20 Comparing control treatment (using OFN yielding 9.88 0.21 g/L·h) indicated procedure creating high levels C. medium.